目的 在体内外研究京尼平苷对葡萄糖异常的调节作用。方法 用ELISA方法测定京尼平苷对高糖诱导的胰腺β细胞胰岛素分泌和细胞活力的影响,同时在链脲佐菌素诱导的糖尿病大鼠体内考察京尼平苷对糖脂代谢的调节作用。结果 京尼平苷可明显降低高糖诱导胰腺β胰岛素分泌、改善高糖诱导胰腺β细胞功能障碍,改善链脲佐菌素诱导的糖尿病大鼠糖耐量、血糖和血脂水平。结论 京尼平苷对葡萄糖异常有明显的调节作用。
Abstract
OBJECTIVE To investigate the regulation of geniposide on blood sugar and lipids in diabetic rats. METHODS ELISA assay was used to determine the content of insulin in vitro and in vivo, and MTT was used to determine the cell viability.RESULTS Geniposide prevented pancreatic beta damage induced by high glucose, and attenuated glucose-stimulated insulin secretion in the presence of 25 mmol·L-1 glucose. Furthermore, geniposide ameliorated insulin resistance, and decreased the contents of total cholesterol (TC) and triglyceride (TC) in STZ-induced diabetic rats. CONCLUSION Geniposide is beneficial to improve the dysfunction of pancreatic beta cells induced by high glucose, and might be a promising lead compound for the treatment of diabetes.
关键词
京尼平苷 /
葡萄糖 /
糖尿病
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Key words
geniposide /
glucose /
diabetes
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中图分类号：
R965
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参考文献
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[7] GUO L X, LIU J H, YIN F. Regulation of insulin secretion by geniposide: possible involvement of phosphatidylinositol 3-phosphate kinase . Eur Rev Med Pharmacol Sci, 2014, 18(9):1287-1294.
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脚注
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基金
国家自然科学基金项目资助(81373459);
重庆市科委重点项目资助(2015jcyjbx0064,2017jcyjB0185);
重庆市科技创新领军人才项目资助(2014kjcxljrc0018);
重庆市杰出青年基金项目资助(2014jcyjjq10003);
重庆市科技创新团队项目资助(CXTDX201601031);
重庆理工大学研究生创新项目资助(ycx2018249)
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